- ￥123640
- Beckman Coulter贝克曼库尔特
- 美国
- C27857
- 2025年07月13日
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文献和实验4) ABTS Liquid Substrate: Aspirate and wash plate 4 times. Add 100μl of substrate solution to each well. Incubate at room temperature for color development. Monitor color development with an ELISA plate reader at 405 nm with wavelength correction set
maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs.Both hydrophobic and ionic interactions stabilize the anionic form of the dye,causing a visible color change.The assay is useful
Fluorochrome Absorption and Emission Spectra
flow cytometer use, we recommend using a 585 ?21 nm BP filter for optimal detection (Fig. 1). When performing multi-color analysis with a dual-laser system, a tighter window of detection is required to compensate for the other conjugates being used (e.g
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