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文献和实验SSR GEL and Silver Staining Protocol
Service Research 5985 Highway K Rhinelander, WI 54501 USA Phone: 715-362-1121 Fax: 715-362-1166 e-mail: pmarquar@newnorth.net I. EQUIPMENT: DNA sequencing unit (35 x 45 cm) & 2000V power supply Clamps Lg. plastic trays (4), about 43 x 50 x
胚胎和成年斑马鱼眼情的组织学准备Histological Preparation of Embryonic and Adult Zebrafish Eyes
1% (w/v) methylene blue 1% osmium tetroxide Propylene oxide Tricaine Zebrafish embryos or adults of interest
Differential cDNA Screening Procedures
for radioactivity. (f.)SIZE FRACTIONATION OF cDNA AND LIGATION TO LAMBDA 1. Save 0.5 ul of the ligation mix in 5 ul of TE with bromphenol blue stop dye. 2. Heat inactivate ligase by heating at 65 C for 10 min. 3. To the ligation mix add: 1O X
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