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文献和实验microscope using a dual filter set for FITC & rhodamine. Cells that have bound Annexin V-FITC will show green staining in the plasma membrane. Cells that have lost membrane integrity will show red staining (PI) throughout the nucleus and a halo of green
FACS Procedures for Apoptosis Detection
Analyze samples. FACS setup: Primary laser: 310nm (UV), 50mW. Secondary laser: 488nm (visible), 250mW. Ho 33258 detection: 424/44 BP filter, Fl-1 detector. 7-AAD detection: 660/20 BP filter, Fl-4 detector. TUNEL
DETECTION OF RECOMBINANT PROTEINS BY ELISA
reader so that a 10 -3 dilution of a "reference serum gives an OD of 1.0 at 405 nm. Continue to read the remainder of the plate. If the ELISA plate reader is not available, stop the reaction by adding 10 µl of 30% NaOH per well. Store at 4°C overnight
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