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文献和实验into one of the V-shaped wells with a pasteur or other type of pipet. The area under the coverslip fills by capillary action. Enough liquid should be introduced so that the mirrored surface is just covered. The charged counting chamber is then placed
) containing 4mM L-glutamate, 4.5 mg/ml glucose and 0.11 mg/ml sodium pyruvate (Invitrogen UK, Cat no: 41966-029), supplemented with 100 U/ml Penicilin and 100 µg/ml Streptomycin (1% v/v Penicilin/Streptomycin solution, Invitrogen UK, Cat no: 15140-122
sure there is a good seal, pressure 75-80 mm Hg for1 hr or more blot membrane dry, cut top right corner wrap in saran wrap and UV irradiate, RNA side down for 2.5 min wash couple min in hot 2X SSPE/0.1% SDS sol'n (microwave 30-45sec, 50% power) air
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