- ￥54164
- Beckman Coulter贝克曼库尔特
- 美国
- B29189
- 2025年07月11日
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文献和实验flask 1-2 times per day Add Colcemid (about 45 min before harvesting) Make 2 aliquots and transfer cell into 50 ml Falcon tubes Incubate in cell cuture incubator or 37°C water bath for additional 45 min Centrifuge for 10 min
expression)(based on Bio Techniques (1999) 26:1042-4)1. Mix 80-160ul of freshly prepared cells with 10-25ug of linearized DNA (DNA in 5-10ul of sterile water) and transfer them to an ice-cold (0 degrees) 0.2cm electroporation cuvette (BIO RAD genepulser).2
should be no higher than 1 ug/ul in the large-scale digest. 2. Extract the large-scale digest once with an equal volume of phenol/chloroform and once with an equal volume of chloroform. Precipitate the DNA with 2.4 volumes of ethanol, spin down and dry
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