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- Beckman Coulter贝克曼库尔特
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- 2025年07月14日
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文献和实验tube. 12) Add 30–100 μL RNase–free water to the center of the spin cartridge. 13) Incubate at room temperature for 1 minute. 14) Centrifuge the spin cartridge for 2 minutes at ≥12,000 × g at room temperature to elute
Preparation and purification of biotin-labeled probe for ISH
Corex tube and insert the packed and washed syringe into the Corex tube in such a way that the tip of the syringe fits into the 1.5 ml tube. Load the nick translation product (55 µl with stop buffer) and spin at 1,500 rpm for 4 min. The probe
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
. It is best to use a rotor–stator homogenizer to homogenize cells for 30 s. If not, pass the lysate at least 5 times through a needle fitted to an RNase-free syringe. 4. Add 1 volume (usually 350 µl or 600 µl) 70% ethanol, mix
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