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- Beckman Coulter贝克曼库尔特
- 美国
- B87475
- 2025年07月12日
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文献和实验Mag-Bind RX Plasmid Isolation Protocol
). Allow the lysate to clear. A tight pellet should form in the corner of each well, which allows the a pipette tip to descend to the center of the well without disturbing the pellet. 8. Transfer 200 ul of cleared cell lysate into another new Process
Gene Knockout Techniques(1) The Knockout Process
without tips remain to have worms added. This requires some care and a deft touch, as it?s easy to eject with such force that the tip jumps back out of the well. Also, removing the tips at the end with a 12- or 8-channel pipetter requires care so that you don?t
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
Plates are treated one at a time and as quickly as possible. Remove medium wash with PBS. Don't wash with PBS/EGTA as this causes the feeders to lift off and the ES cell colonies to float away and break up. Add a small volume of PBS to plate, hold plate
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