- ￥38115
- Beckman Coulter贝克曼库尔特
- 美国
- A19793
- 2025年07月12日
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文献和实验Preparation and purification of biotin-labeled probe for ISH
, however the size of the probe is relatively easier to control by nick translation. II. Purification of the biotin-labeled probe. Start 20 min before the end of the nick translation incubation. Plug the bottom of a 1 ml tuberculin syringe
(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
Sucrose Fractionation of Castor Bean
), attach a 2.0 ml syringe and pull out the bottom 1.5 ml of the sample. Remove the syringe and place the 1.5 ml fraction into a test tube marked as #1. Re-attach the syringe without disturbing the gradient, and repeat as often as need to totally fractionate
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