- ￥44000
- Beckman Coulter贝克曼库尔特
- 美国
- C28420
- 2025年07月12日
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文献和实验the plasmid DNA of interest into a 17 X 100 mm Falcon tube containing 6 ml of TB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 16-18 hours with shaking at 250 rpm. 2. Harvest the cells
microscope. This will allow observation of the cells without opening or disturbing the growth. 16. Make cell density determinations at 10 X magnification using a square ocular grid, as explained in Chapter One for the determination of area. 17. Plot
CSF Extract Prep for Spindle Assembly
pipettes (diameter of opening approx. 2-3 mm) LPC (10 mg/ml each of leupeptin, pepstatin, chymostatin in DMSO) Cytochalasin D (10 mg/ml in DMSO) 13 X 51 mm ultraclear tubes SW55.1 @ 16 deg C in ultra Procedure
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