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文献和实验polymerase. After the reactions are extended for 2 minutes at 67℃ and briefly centrifuged, four aliquots are removed and added to the appropriate base-specific termination mix. All nucleotide mixes contained the guanosine nucleotide analog, 7-deaza-dGTP
]dATP or [a-35-S]dATP and Bst polymerase. After the reactions are extended for 2 minutes at 67deg C and briefly centrifuged, four aliquots are removed and added to the appropriate base-specific termination mix. All nucleotide mixes contained
2 Dimensional Gel Electrophoretic Analysis for Chicken Egg
contents of the chicken egg 2. IPG strip 3. Buffer solutions: Lysis buffer: 50 �l 9.5 M urea + 0.0038 g DTT + 40 mM Tris base + 2% NP-40 IPG buffer: 5 �l Sample buffer: ? �l 8.3 M urea + 2 M thiourea + 4% NP-40 + 1 mg/L bromophenol blue
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