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文献和实验of containing most of the necessary reagents pre-packaged in RNase-free quality (e.g. polyATtract from Promega or Oligotex mRNA kit from Qiagen work well for Neurospora, Table 1)。 Depending on the kit size, this allows isolation of poly(A)-RNA from 0.25 to 5 mg
1.1. Can the NCIS Kit be used on adults? The NCIS kit is specifically packaged for five separate tissue dissociations, each containing up to twelve neonatal rat hearts. The NCIS protocol is available online at www.worthington-biochem.com
Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
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