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文献和实验shearing causes foaming of the lysate due to detergent in the buffer, however, this should not effect the mRNA yield. The foam can be reduced by a 30 second centrifugation. The lysate can be frozen and stored at –80°C for later use. v
Native Chromatin Preparation and Illumina/Solexa Library Construction
) Incubate at room temperature for 45 min. 40. Use MinElute PCR Purification Kit or phenol:chloroform extraction to purify DNA. Elute or resuspend DNA in 30 µL of 1X TE, pH 7.4. 41. Add "A" to 3'' ends
Introduction to the EMSA (Gel Shift) Technique
by the relatively low ionic strength of the electrophoresis buffer used. Upon entry into the gel, protein complexes are quickly resolved from free DNA, in effect freezing the equilibrium between bound and free DNA. In the gel, the complex may be stabilized
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