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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
cell isolation kit) per 107 cells. 12. Mix well and incubate for 15 min at 4°C-8°C. 13. Wash cells with T cell isolation buffer by adding 10X-20X the final labeling volume. Centrifuge at 300g for 10 min. Pipette
(left-over from the extraction procedure) can inhibit PCR by reducing Taq polymerase activity to as low as 10%, however, inclusion of 0.5% Tween-20 or -40 will effectively neutralise this effect (Gelfand 1989). TMAC is generally used at a final
Differential cDNA Screening Procedures
rice leaves 10 ul (10 ug poly(A)+RNA) 3.0 M NaOAc 1.0 ul Ethanol 25 ul Chill at -20 C, spin down RNA pellet. Dry down in a speed vac dessicator. 3. Resuspend in 10-ul of primer. 4. Heat to 65 C for 5.0 min. 5. Quick chill
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