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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
from the reservoir). 5) Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head
Hybridization of High Density Arrayed BAC Nylon Filter Blots
Protocol for hybridization of high density arrayed Bacterial Artificial Chromosome nylon filter blots with 100 PCR isolated Unigene cDNA inserts, pooled in a combined probe Isolation and purification of cDNA inserts using polymerase
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