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文献和实验High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining
. Yeh, C.G., His, B., and Faulk, W.P. 1981. Propidium iodide as a nuclear marker in immunofluorescence. II: Use with cellular identification and viability studies. J. Immunol. Methods 43:269‐275
Solutions 70% ethanol ribonuclease (100 µg/ml DNase free, Sigma) propidium iodide ( 50 µg/ml in PBS) Procedure Harvest cells. Spin at 1200 rpm for 5 minutes. Discard supernatant; add 1 ml ' fridge
A general protocol for staining cell for cytometry analysis
(Cat. No. 66121A) : 0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1X prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1X PBS buffer. Store at RT. Recommended for use in parallel with Annexin
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