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- Beckman Coulter贝克曼库尔特
- 美国
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- 2025年07月11日
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
to 37°C and 65°C Sonicator Spectrophotometer Thermal cycler, automated Timer Tubes, microcentrifuge, 1.5-mL Tubes, polypropylene, 14-mL (e.g., 17- x 100-mm) for use in high-speed centrifuge Tubes, polypropylene, 50-mL
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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