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- Beckman Coulter贝克曼库尔特
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- M2P-E-OP-432
- 2025年07月06日
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文献和实验Solutions 70% ethanol ribonuclease (100 µg/ml DNase free, Sigma) propidium iodide ( 50 µg/ml in PBS) Procedure Harvest cells. Spin at 1200 rpm for 5 minutes. Discard supernatant; add 1 ml ' fridge
with an expression vector encoding the gene of interest. Subsequently cells are stained with propidium iodide and, utilising flow cytometry, transfected, GFP-expressing single cells are detected and apoptotic cells in this population are identified by their DNA
processor plus module, permitting the doublet discrimination analysis. The software used was CellQuest Pro (BD Biosciences). Nuclei were counterstained with 250 µg/ml propidium iodide (PI; Sigma–Aldrich). Single nuclei were gated on the basis of PI staining
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