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文献和实验E.Z.N.A.® Mag-Bind® Viral DNA/RNA Spin Protocol
3. Glycogen 0.8 ml 2 x 1.5 ml 4. Buffer VHB 34 ml 2 x 66 ml 5. SPR Wash Buffer 4 x 12 ml 4 x 20 ml 6. Nuclease free Water 10 ml 45 ml 实验设备 Purification 1 x 96 Preps 4 x 96 Preps 实验步骤
Gene Knockout Techniques(1) The Knockout Process
column pool DNA, and a small amount from these reactions is used to inoculate the internal primer reactions. Each 384-well plate will accommodate 96 reactions for each of four different screening intervals. Assemble a master mix of sufficient
more Proteinase K during the tail digestion. From an average tail piece of about 1.0 cm, we usually get about 200 µg or so, thus for restriction digestion, we usually use 10 µl. Add 10 µl from each tube to wells in a round-bottom 96 well microtiter plate
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