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文献和实验Construction and Characterization of Adenovirus Vectors
vi. Centrifuge cells at 650g for 20 min at 4°C. Decant the medium and retain the cell pellets. vii. Resuspend the cells in 3 mL of 4% sucrose. Transfer to a 50-mL conical tube. viii. Using
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
to 37°C and 65°C Sonicator Spectrophotometer Thermal cycler, automated Timer Tubes, microcentrifuge, 1.5-mL Tubes, polypropylene, 14-mL (e.g., 17- x 100-mm) for use in high-speed centrifuge Tubes, polypropylene, 50-mL
Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
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