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文献和实验E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues
min at 5000 x g to completely dry the HiBind® matrix. 7. Elution of RNA. Transfer the column to a clean 15 ml centrifuge tube (Not supplied) and elute the RNA with 250-500ul of DEPC-treated water (supplied with kit). Make sure to add water
) for 1 minute to dry the column. 8) Place the column in a clean 1.5 mL microcentrifuge tube and add 10-20ul Elution Buffer (10mM Tris, pH8.5 ). Stand for 1-2 minute and centrifuge at maximal speed ($13,000 x g) for 1 minute to elute DNA.
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
-through. Then with the collection tube empty, centrifuge the spin cartridge at 10,000 x g for 2 min at room temperature to completely dry the HiBind® matrix. 11. Transfer the column to a clean 1.5 ml micro centrifuge tube (not supplied with kit) and elute the RNA with 30-50
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