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文献和实验E. Z. N.A.TM X-press Plasmid Protocol
1.5 and 2.0 mL Centrifuge Tubes 3. Vortex 实验步骤 1. Pick up a single colony from a fresh streaked selective plate and Inoculate 2-3 mL LB medium containing the appropriate selective antibiotic. Incubate 14-16 hours at 37℃
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
containing the antibody/agarose complex. Mix by flicking tube gently. (36) Incubate at RT for 15 min. (37) Pellet agarose by brief centrifugation (3000-5000 x g (6600 rpm) for 1 min), and collect supernatant into new tubes. (38) Repeat steps 35
E-Z 96 X-press Plasmid Vacuum Protocol
实验试剂 Materials to Be Provided by User 1. Nuclease Free 1.5 and 2.0 mL Centrifuge Tubes 2. Ice 3. Isopropanol (100%) 实验设备 Materials to Be Provided by User 1. Tabletop
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