Cs, Tips, 40 µL, Sterile fltrd

Single tube confirmation PCR protocol

(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants.   2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution

Embryo Dissection and Micromanipulation Tools

  Embryo Dissection and Micromanipulation Tools Hazel L. Sive , Robert M. Grainger , and Richard M. Harland Adapted from "Equipment for Embryo Experiments," Chapter 4, in Early Development of Xenopus laevis by Hazel L. Sive, Robert M

Protocol for competitive RT-PCR

, factor 2-2.5 between the standard amounts gives more accurate results (i.e.: 25 pg; 50 pg; 100 pg; 250 pg). In the following PCR 3-5 µl of cDNA, 1.5 units Taq DNA polymerase (Pharmacia), 200 µM of each dNTP, 250 nM of each primer and 1/10