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- Beckman Coulter贝克曼库尔特
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- 2025年07月12日
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文献和实验Clonality - X Chromosome Inactivation Assay
of the resuspended DNA into a reaction tube. Add 1 µl REact buffer 2. Add 1 µl Hha I. Incubate overnight at 37°C. Incubate at 90°C for 10 min. Use 2 µl of this digested DNA for PCR (see below). Use 2 µl of the non-digested DNA from B18 above as a negative
Adult X. tropicalis in our stock lines are generally a uniform brown color, with lighter patches appearing often near the nose (see inset). The female (bottom, main panel) is larger than the male (upper, main panel). The animals have eyes
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
by inverting the tube a few times. Incubate 5 minutes at room temperature. Invert the tube several times during incubation. NOTE: ERL Buffer is supplied as a 10x concentrate and must be diluted with ddH2 O according to bottle label. 2. Centrifuge
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