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- Beckman Coulter贝克曼库尔特
- 美国
- C16675
- 2025年07月14日
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文献和实验Large Scale Plasmid Preps: PEG method
1. Grow 250 500 mL of bacteria overnight in LB with 50 µg/mL of ampicillin. 2. Transfer culture to Nalgene bottles. Spin in SGA rotor at 6000 rpm, at 4°C for 10 min. 3. Discard supernatant. Keep everything on ice. Resuspend
to hydrolyze DNA, using an RNAse free DNAseI, and purify the cleaned RNA free of DNA and free of DNAseI activity.ProcedureTransfer the total RNA to a clean 1.5 ml tube and adjust the volume of the total RNA sample to 100 ul with RNAse free water.Add 350 ul
2. Grind 2 g of fresh, leaf tissue to a powder in Liquid Nitrogen in a chilled mortar and pestle. 3. Scrape the powder into a chilled 50 ml tube. 4. Add 3 to 5 ml CTAB Buffer per gram tissue to the tube. 5. Incubate the sample at 60℃
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