819/44nm Bandpass Filter

A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transiently transfected cells

for eGFP, 610 nm longpass filter for PI, 675/20 nm bandpass filter for Cy5 and a 424/44 nm bandpass filter for DAPI. Doublets were excluded by pulse processing. Cells expressing eGFP were gated and analysed for either Cy5- or PI-fluorescence. Data

Sucrose Fractionation of Castor Bean

Materials Castor beans       Gradient former Sucrose solutions in 0.01 M EDTA, pH 7.5       33%, 44%, 50%, 57%, 60% (w/v)        Grinding Medium             0.4 M sucrose             0.165 M Tris-HCl buffer, pH 7.5             0.01 M KCl          

Quantum Dots for Quantitative Flow Cytometry

is the quantum yield of the fluorophore, and %T is the percent fraction of emitted light that is transmitted by the bandpass filter at the detector PMT. The modular construction of beads decorated with discrete quantities of quantum dots with defined