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- Beckman Coulter贝克曼库尔特
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- 2025年07月12日
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文献和实验Purification of Plasmid from 50 ml-culture
2. Spin the bacterial culture at 6,000 rpm for 10 min at 4 C. Discard the supernatant. 3. (0ptional) Suspend the cell in about 20 ml of deionized water. Spin again. Discard the supernatant. Remove all of the supernatant fluid using pipetman.
Purification of Plasmid from 50 ml-culture
.) 2. Spin the bacterial culture at 6,000 rpm for 10 min at 4 C. Discard the supernatant. 3. (0ptional) Suspend the cell in about 20 ml of deionized water. Spin again. Discard the supernatant. Remove all of the supernatant fluid using pipetman
AMNIOTIC FLUID CULTURES ON COVERSLIPS
. Resuspend pellet in media (4 coverslips, add 1.5 ml ; 3 coverslips, add 1.0 ml of media). Add 0.5 ml to each coverslip, being careful to keep it on the coverslip. Do the same for each tube of amniotic fluid. Set-up day is day 0. Place "A" coverslips
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