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文献和实验AMNIOTIC FLUID CULTURES ON COVERSLIPS
ml conical centrifuge tubes. If the sample is received differently, transfer to two tubes. Centrifuge the tubes for 6-8 minutes at 150 x g (900 rpm). Leaving 0.5 ml, transfer supernatant to a 15 ml centrifuge tube (this will be frozen for back-up AFP
Fibronectin Sigma F0895-2MG Glass Bottle (250ml) with Cap Cole Parmer Instrument Co. 34594
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
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