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文献和实验ASC Speck Formation as a Readout for Inflammasome Activation
All inflammasomes require the adapter protein apoptosis associated speck-like protein containing a CARD (ASC) for the activation of caspase-1. After inflammasome activation, ASC assembles into a large protein complex, which is termed “speck
AFLP: not only for fingerprinting, but for positional cloning
to another but is of a single, defined sequence within a given primer. The selective nature of AFLP-PCR is based on the 3' extensions on the oligonucleotide primers. Since these extensions are not homologous to adapter sequence, only plant DNA fragments complementary
AFLP: not only for fingerprinting, but for positional cloning
. Since these extensions are not homologous to adapter sequence, only plant DNA fragments complementary to the extensions will be amplified due to the inability of Taq DNA polymerase, unlike some other DNA polymerases, to extend DNAs if mismatches occur at the 3'-end
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