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文献和实验Purification of recombinant sBRF M166L
will decrease the foam in the culture during growth). Incubate at 30 deg till the culture reaches OD600 ~ 0.6. Remove a small 1 ml sample for an uninduced control, and incubate this small sample on the tube roller for an additional 4 hrs. Induce the large
Purification of recombinant sBRF M166L
will decrease the foam in the culture during growth).Incubate at 30 deg till the culture reaches OD600 ~ 0.6.Remove a small 1 ml sample for an uninduced control,and incubate this small sample on the tube roller for an additional 4 hrs. Induce the large culture
LABELING ALPHA-ACTININ WITH IATR
Materials 1.Small vial and stir bar. 2.Bio-Beads SM-2 in 0.7x15 cm column. 3.2 mM Tris-HCl,pH 8.5,4℃,250 ml. 4.Centricon-30 concentrator. 5.5 mM Tris-acetate,0.1 mM DTT,pH 6.95,4℃,250 ml. 6.200 mM K-borate,pH 9.0,10 ml. 7.DTT,100 mM stock
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