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文献和实验RNA AND PROTEIN EXTRACTION FROM THE SAME SAMPLE
-HCl pH 8. Store in dark bottle at 4o C for 1-4 month 3. Phenol: Chloroform: Isoamyl alcohol (PCI) (25:24:1) (50 mL): Mix 25 mL of equilibrated saturated phenol, 24 mL of chloroform and 1 mL of isoamyl alcohol. Store
Summary: Illumina's Solexa Sequencing Technology
with single stranded oligonucleotides that correspond to the sequences of the adapters ligated during the sample preparation stage. Single-stranded, adapter-ligated fragments are bound to the surface of the flow cell exposed to reagents for polyermase-based
& top, remove the filter from its packaging and screw it onto the bottle. The filter is sterile, so try not to touch it to anything else Attach the adapter to the filter and the vacuum nozzle to the adapter (vacuum should be off) Pour the CSM
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