- ￥8162
- Beckman Coulter贝克曼库尔特
- 美国
- B30156
- 2025年07月08日
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文献和实验,000 x g (9000 rpm)for 10 min at 4℃ in a SS-34 rotor. 5.In the cold room,collect the supernatant,transfer it to 31.5 mlμltracentrifuge tubes,pair them by weight,and clarify the cytosol by centrifugation at 100,000 x g (29,000 rpm)for 60 min at 4℃
debris by centrifugation at 10,000 x g (9000 rpm)for 10 min at 4℃ in a SS-34 rotor. 5.In the cold room,collect the supernatant,transfer it to 31.5 ml μltracentrifuge tubes,pair them by weight,and clarify the cytosol by centrifugation at 100,000 x g (29
Preparation of Rat Liver Cell Cytosol
in slushy ice, homogenize with a Teflon pestle by 6 slow passes at 3,000 rpm. Transfer the homogenate to 31.5 ml centrifuge tubes, and remove cellular debris by centrifugation at 10,000 x g (9000 rpm) for 10 min at 4oC in a SS-34 rotor.
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