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文献和实验Rotor equilibration without counterbalance
Rotor equilibration without counterbalance Contributed by Dr.A.Gratchev This is relatively obvious method, which I found not used by most of the researchers. Usually the equilibration of 2,3,4,6,8,9, and 10 tubes in 12x rotor
detection Reagents: Formamide (FA) / 2x SSC (1:1) 0.1x SSC 4x SSC / 0.1% Tween20 3% BSA in 4x SSC / 0.1% Tween 20 DAPI (4.6-diamidino-2phenylindole dihydrochloride): stock solution 0.2 mg/ml, dissolve 1:5000 in aqua bidest
and other parameters will vary with the particular instrument and rotor used. Relative Centrifugal Force is a constant that is independent of the apparatus used. Figure F.2 presents a Nomogram for calculation of R.C.F. for a given radius and RPM. A simple formula
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