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- Beckman Coulter贝克曼库尔特
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- 2025年07月12日
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文献和实验LABELING GIZZARD MYOSIN WITH IATR (MHC AND 17KD MLC)
(injection buffer). Procedure 1.Pellet myosin at 15,000 rpm,4℃,for 10 min in a SS34 rotor. 2.Soak and resuspend pellet in ~200μl of buffer 1.Handle gently. 3.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2Ti rotor. 4.Determine myosin concentration using
Sucrose Density Gradient Fractionation of Yeast Membranes
stock in SK at -20oC) at room temp, then spin 1 min at 14,000 rpm. Take supe.-- for 4 gradients, use 900 ul of supe, 90 ul of beta-mercaptoethanol and bring to 45 ml with SK (this recipe should be adjusted for the appropriate number of gradients--we want
of beta-mercaptoethanol and bring to 45 ml with SK (this recipe should be adjusted for the appropriate number of gradients--we want to conserve zymolyase--it's expensive) Shake gently, 45 min, 60 rpm, 30oC
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