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文献和实验the AllPrep DNA/RNA 96 Kit. Six targets (195-1150 bp) were amplified by multiplex PCR and then analyzed on the QIAxcel system. Li: liver; Ki: kidney; Lu: lung; In: intestine; Sp: spleen; NTC: no template control (shows upper and lower markers). Total
for 60 minutes. 5) Add 100 μl FP3 Buffer and vortex to mix. 6) Store the extraction at 2-8°C. 2. PCR Protocol This protocol serves as a guideline for PCR amplification. Optimal reaction conditions, such as incubation times
is covered by the Extraction Solution. 2) Incubate at 56°C for 1 hours. 3) Incubate at 95°C for 5 minutes. 4) Add 50 -100 μl PS3 Buffer and vortex to mix. 5) Store the extraction at 2-8°C. 3. PCR Protocol
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