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文献和实验volume)Day 11.Inoculate 5mls YPD (in a 50 ml tube) with yeast strain (single colony on YPD plate or scraping from glycerol stock). Incubate O/N at 28-30 degrees, shaking.Day 22.In early afternoon (3pm), inoculate 100mls of YPD (500ml flask) with 100ul of O/N
Preparation of NeoR Murine Embryonic Fibroblasts
for 10 more minutes. Put all the contents of the dish in a 50ml conical tube. Allow the pieces of cellular debris to settle out over a period of 2 minutes. Remove the supernatant into a fresh tube, bring the volume up to 50ml with MEF media and spin
Preparation of NeoR Murine Embryonic Fibroblasts
to a heterozygote female. 13.5 days to 14.5 days after the plug is observed, sacrifice the pregnant female either by cervical dislocation (after appropriate anesthesia) or CO2 asphyxiation. Prepare three 10cm dishes containing 15ml PBS each. With the mouse
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