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- Beckman Coulter贝克曼库尔特
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- 2025年07月15日
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文献和实验Preparation of BAC DNA with by Alkaline Prep Method
minutes and then use horizontal rocking to dissolve pellet. Alternatively, the 10T/50E solution could have been pre-warmed to 37℃and used to dissolve the pellet. DO NOT VORTEX AT THIS STEP!!!!!!!! Transfer the DNA solution to a 50ml capped tube by pouring
Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
, use 184.03g into 250ml autoclaved MilliQ H2O, store at room temperature) 50ml Glacial Acetic Acid 23ml Water 127ml 8. Spin at 15,300 xg RCFMax (10,000 rpm Beckman JA14 rotor; 10,000 rpm Sorvall SLA-1500 rotor) for 15
culture (glycerol stock the rest).Day 33.Spin cells down in 2 x 50ml tubes (1500g for 5 min at 4 degrees; JA10 rotor 2900rpm)4.Resuspend pellets in 10mls Buffer A; incubate at 30 degrees for 15 mins (NO SHAKING, place tubes in 30 degree incubator
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