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文献和实验Double dye filling (using DiO and di-4 ANEPPS)
Double dye filling (using DiO and di-4 ANEPPS) (Krista Williams) Dye Filling Label 15 ml centrifuge (c/f) tubes with strain name. Squirt ~1-2 ml M9 onto plate with a Pasteur pipet. Rinse plate with M9 using a pasteur pipet
from the genetic mutation is characterized. Core personnel are available for consultation on all aspects of gene targeting research. Contact Thom Saunders with any questions or Meet the Staff. The Transgenic Core prioritizes all requests for service
-Aldrich Technical Service. It is not necessary to use calcium free buffers to dilute the stock solution because the BAPTA-AM will not bind any calcium until the AM is cleaved off by esterase activity within the cell. I hope
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