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- Beckman Coulter贝克曼库尔特
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文献和实验for 10 minutes at 500 x g. 9. Wash again by adding 200 µl TE pH 7.5 and concentrating to about 20-30 µl (approximately 8-10 min at 500 x g). Alternatively, a smaller pore MicroCon 30 can be used to speed the concentration step. In this case
Gene Knockout Techniques(1) The Knockout Process
to save time and materials, but everything can be done in 96-well plates if you don?t have access to 384-well equipment. 10 ul reactions work fine in 96-well plates, and don?t require nearly as much Taq. To date we have done most steps with worms grown on solid
Lacrimal Experimental Protocol (4/4/94)
6 for 6 min on Eppendorf centrifuge. Retain supernatant and store at -70°C. Make up trypsin/EDTA/dispase by adding 0.9 ml of 10 x trypsin/EDTA to 3.6 ml of dispase; put at 37°C 9. After 100 min, remove and retain stimulation media. Add 100 µl
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