- ￥200
- Beckman Coulter贝克曼库尔特
- 美国
- 870690
- 2025年07月06日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海博耀商贸有限公司
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008）Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
is complete, about 5-20 minutes. 8. Remove lysate filtrate collection block from manifold. Add 2ul of glycogen type II (Sigma Inc.) at 1ug/ul and ice-cold isopropanol (0.7x volume of filtrate) to each well. Seal with plastic sealing tape and mix. Incubate
the collection rack with clean 1.5 ml screw-capped tubes into the manifold. Clamp the manifold with the white clamps, and apply 300 ul of 10:1 TE buffer heated to 65degC and pull the eluted DNA through at 5 in. Hg. After the liquid has filtered through, raise
技术资料暂无技术资料 索取技术资料
