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文献和实验Modified Eberwine (antisense) RNA Amplification Protocol
gel tubes). Pass over ChromaSpin TE+30 column (Clontech #K1321-2). Prepare columns while performing the organic extraction: remove top cap then snap off bottom; prespin 5 minutes at 700 x g, room temperature. When loading column, be careful
the next pad; its thin coating of agarose will make the pad stick to it instead of the fresh top coverslip. Place the coverslips in a box and cover with aluminum foil to dry. Can leave out on the bench overnight, or bake in an oven at 65・
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
Wash in PBS at room temperature for 5 minutes. Detection of the Immunostaining Add 20µl of the diluted Cy3-Anti-Rabbit secondary antibody (1/700e in blocking solution + 2% normal goat serum). Cover with a coverslip and incubate
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