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文献和实验Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
实验步骤 1. Place a empty, uncapped 50 ml conical tube into a 50ml tube holder. 2. Add 1-6 ml blood sample to 50ml tube. 3. Add indicated volume of PBS, MSL, Proteinase K and RnaseA showed in the table to the sample. Mix
Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)
实验步骤 1. Place two empty, uncapped 50 ml conical tubes into a 50ml tube holder. 2. Equally divide the blood sample and transfer the sample into twp 50 ml tubes. For example: For 7ml blood, transfer 3.5ml blood into each tube
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