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文献和实验Supercritical Fluid Extraction of Polychlorinated Biphenyls from Fish Tissue
are typically toxic or flammable and ultimately must be disposed of as hazardous waste. Traditional solvent extracts obtained require multiple postextraction purification steps, such as gel permeation chromatography (GPC), florisil, and silica column clean
of deoxyribonucleic acid (DNA). These methods are performed in closed-tube format, thereby reducing the risk of contamination between stages. In addition, post-PCR processing, such as clean-up steps and gel electrophoresis, are eliminated as the results are read
from the vertical (such as the Sorvall SS-34 rotor or the Beckman JA-20). Figure F.1 presents a cross- sectional diagram of the Sorvall SS-34. Swinging bucket rotors (also known as horizontal rotors) have the advantage that there is usually a clean meniscus
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