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文献和实验Green lab protocol for vacuum infiltration transformation of Arabidopsis
of mercury but the vacuum is not still being directly pulled) and let the plants stay under vacuum for five minutes. Quickly release the vacuum. Briefly drain the pots, place them on their sides in a tray, cover the tray with plastic wrap to maintain humidity
E-Z 96 X-press Plasmid Vacuum Protocol
after incubation. 7. Assemble the vacuum manifold according to the manufacturer’s instruction. For using Omega Bio-Tek’s Vac-03: 1) Place E-Z 96 Xpress Plate on the top plate of manifold; 2) Place a waste collection tray or 2 ml deep
Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
5, was used for transformation by vacuum infiltration. Construction of pGKB5 A new plant transformation vector was designed, that could be used for T-DNA tagging in Arabidopsis. The T-DNA region of this plasmid is flanked by fragments containing
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