- ￥200
- Beckman Coulter贝克曼库尔特
- 美国
- 803027
- 2025年07月16日
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
from the reservoir). 5) Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
scissors and a #3 size scalpel handle Phosphate buffer saline (PBS) Sterile medium size petri dishes (tissue culture standard) 18 gauge needle luer lock syringe (about 6cc should suffice) #11 size flat-edged scaple blade trypsin
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