Adapter, Bottle, 500 mL, Set o

Dropout plates for yeast

  Materials (Solutions are all available from the media room) 200ml bottle of 2x SD 200ml bottle of 4% agar -- make sure to sign it out 40% glucose CSM minus the relevant marker(s), as powder Stack

AMNIOTIC FLUID CULTURES ON COVERSLIPS

Glacial Acetic Acid: FISHER cat # A38-500. Acetic acid, Glacial ACS (Aldehyde free), 500 ml bottle.   L-Glutamine: GIBCO cat# 320-5030. L-Glutamine solution 100X (200 mM), 20 ml bottle, store frozen.   Methanol

Methods for DNA isolation

for 20 minutes in 500 ml bottles in the RC5-B using the GS3 rotor. Resuspend the cell pellets in old media and transfer to two bottles, centrifuge as before, and decant the media. The cell pellets can be frozen at -70degC at this point. 3. Resuspend