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文献和实验Protocols for ET recombination
synthesis, the EMBL oligo service prefers that the 5' most nucleotide is a T or C. Consequently most of our oligos start with a T or C however we do not think this is important for ET cloning efficiencies). The 3' end (the PCR primer) - choose about 18-30
Protocols for ET recombination
about 50) nts for the homology arms from the target DNA sequence simply according to where you want to insert the PCR fragment. (For efficient oligonucleotide synthesis, the EMBL oligo service prefers that the 5' most nucleotide is a T or C. Consequently
Protocols for ET recombination.
synthesis, the EMBL oligo service prefers that the 5' most nucleotide is a T or C. Consequently most of our oligos start with a T or C however we do not think this is important for ET cloning efficiencies). The 3' end (the PCR primer) - choose
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