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文献和实验of 7 x 2 x 0.5" and bottom dimensions of 7 x 2 x 0.25". 2) Recess the top plate in the center portion to 0.125" deep, leaving 0.950" per end for 1.375" wide x 0.3125" deep fluid reservoir. 3) Drill 1/16" wide x 0.625" long
cells (hESC) are difficult to adapt to 96?well plate assays, such as the MTT assay, because they survive best when plated as colonies, which are not easily counted and plated accurately. Two methods were developed to address this problem
Cosmid Cloning: Cell preparation, DNA packaging, and Cell Transfection
To store library, pour 3 ml LB onto plate and scrape colonies into the broth with sterile razor blade. Resuspend cells, pool, add appropriate antibiotic to cell suspension, and freeze aliquots at -80 C.
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