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文献和实验Extraction of DNA using DNAzol® Reagent
. Use a loosely fitting homogenizer, with a tolerance of 0.1-0.15 mm or higher. Homogenize 25-50 mg tissue in 1 ml of DNAzol® Reagent by applying as few strokes as possible. Typically, 5-10 strokes are required for complete homogenization. Small amounts
Antibody to an equal amount of Taq DNA polymerase units. ForTaq DNA polymerase at 5 unit per μl, prepare a 1:1 mixture. Mix well using a Vortex Mixer. 3. Add the following components to a sterile 0.5-ml microcentrifuge tube:
ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
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