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文献和实验Dorsal Lip Transplant in Amphibian Embryos
in any kind of tissue. They used this information to induce the growth of a second neural tube on an already developing embryo. This experiment attempts to repeat Spemann and Mangold's experiment in Xenopus embryos. Detailed protocol 1. The operation
Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
into each microcentrifuge tube (~500 islets/tube/mouse). Allow islets to settle to the bottom of the microcentrifuge tubes. Draw one islet pellet into a P200 pipetman (set at 130ul) using a straight thin-wall pipet tip. Place a silicone tube adapter
of minimum area. In a fixed angle rotor, the materials are forced against the side of the centrifuge tube, and then slide down the wall of the tube. This action is the primary reason for their apparent faster separation, but also leads to abrasion
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