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文献和实验35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION
Pipet 12.5µl 35S-UTP (1200 Ci/mmol) into 1.5ml microfuge tube. Final concentration should be 12µM. Lyophilize in speed vac. (Do not use 35S-UTP that has been previously thawed. Order 250µCi vials and use up at first thawing or discard
35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION
35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION by Josiah N. Wilcox, Ph.D. (based on Melton et al., 1984) Pipet 12.5µl 35S-UTP (1200 Ci/mmol) into 1.5ml microfuge tube. Final concentration should be 12µM. Lyophilize
by centrifugation x 5 min at 600g and resuspend in 5 ml of EPC medium. 6. Cell seeding of slide prior to flow chamber assembly 1) A slide made out of glass, titanium, or other material can be used, and the slide surface can be modified by spin
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